Cellular Response Technologies (CRT)

CRT fees

Effective: July 1, 2024

Services

Internal IU Faculty rates

External rates
Ficoll Preparation of Human Blood MNCs (20mls)
  • Preparation includes underlaying of up to 20mls of blood, washes and cell count
$62$93
CPT Preparation of Human Blood MNCs
  • Preparation includes processing of up to 48mls of blood in CPT tubes (supplied by the user), washes and cell count
$88$131
RBC lysis of human or murine tissues (per tissue)
  • Preparation includes lysis, washes, and cell counts
$12$18
Plasma Separation and Freeze Down
  • Preparation includes centrifuging and freeze down of up to eight vials of plasma
$23$35
MNC Separation and Freeze Down (with 7C Flow)
  • Preparation includes freeze down of up to five vials of MNCs
$20$30
MNC Separation and Freeze Down (without 7C Flow)
  • Preparation includes isolation of MNCs and freeze down of up to five vials of MNCs
$87$131

ServicesInternal IU Faculty ratesExternal rates
7-Color MPFC Analysis of Human Blood MNCs
  • Staining of MNCs with seven antigen panel (CD14, CD16, CD31, CD34, CD45, AC133, LIVEDEAD), including necessary FMO controls and viability markers, and brief analysis in FlowJo
  • Does not include cost of data collection in Flow Cytometry Core
  • Isolation of MNCs is a separate charge
$200$300
Up to 14-Color MPFC Analysis of Human Blood or murine tissues

Can design any panel for the specific PI's interests
  • Staining of MNCs with eight antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo.
  • Does not include usage charge from Flow Cytometry Facility
  • Isolation of cells is a separate charge
$254$381

ServicesInternal IU Faculty ratesExternal rates
Quantitation of CFU-GEMMs from Human Blood
  • Plating, culture and quantitation of CFU-GEMMs (in triplicate)
  • Isolation of MNCs is a separate charge
  • Blood provided by Investigator
$54$81
Quantitation of ECFCs)from Human Blood
  • Plating, maintenance and quantitation of three wells for ECFCs
  • Isolation of MNCs is a separate charge
  • Blood provided by Investigator
$340$509
Expansion and Cryopreservation of ECFCs
  • Expansion of ECFC colonies to passage 2 and cryopreservation of cells
  • Blood provided by Investigator
$297$446

ServicesInternal IU Faculty ratesExternal rates
Tube Forming Assays (Matrigel) with Human Endothelial Cells
  • Up to 10 conditions in triplicate assayed for endothelial cell tube formation
  • Does not include cost of cell line
  • Does not include Incucyte use
$261$392

Proliferation, Migration, Invasion Assay on various cells
Culture, and plating of cells for various assays
*Investigator must provide cell line

TBDTBD

ServicesInternal IU Faculty ratesExternal rates
Charge per 1 plate/day$48$72

ServicesInternal IU Faculty ratesExternal rates
Cell Characterization, FCCP Titration$185$278
Cell Glycolysis Assay-Stress Test$214$322
Mitochondrial Respiration-Mito Stress Test$195$293
Cell Energy Phenotype Assay$171$256
Mito Flex Fuel Assay$211$316
Training for Equipment Use$290$435
*The price of the assays include the cost of the Seahorse consumables and kits without any overhead charge.

ServicesInternal IU Faculty ratesExternal rates
Technician time$40/hour 
Equipment Fee$25 $37
Retrospective analysis of FCS3.0 files
  • Data mining/analysis/re-analysis of previously acquired list mode data (FCS3.0 files) of EPC or CHSPC phenotyping for newly identified cell
    sub-populations of interest
$52/hour $78/hour

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