Learn more about the science of flow cytometry
These tutorials, references, and publications have been chosen to help you learn about flow cytometry or expand your existing knowledge of the field.
Vendor resources, training, and tutorials
Fluorofinder
A resource to design panels and view spectral characteristics of any fluorescent dye. After creating an account, you can go to the account settings and select “Indiana University” under institution and “Simon Cancer Center Flow Cytometry (Indianapolis)” under Facility. This will allow you to view and select the exact instruments available at the Flow Core.
FlowJo Analysis Software
One of the leading flow cytometry analysis software that allows users to import data from the conventional (BD Fortessa/X-20) and full-spectrum (Cytek) instruments, run compensation/unmixing and analyze the FCS3.1 files on your own computer. For a license, please contact Tina Hedges (klhedges@iu.edu). This software is also available for free on two computers in the flow core on a first-come-first-serve basis.
Image Stream
Resources for the imaging flow cytometer including applications, products and manuals.
Cytek Biosciences
Website dedicated to the Cytek Aurora spectral analyzer. Loaded with tutorials, protocols, and more to get you familiar with spectral cytometry.
BD Biosciences Flow Cytometry Tutorial Videos
A great resource that covers flow cytometry basics as well BD biosciences instrumentation and software
BD Biosciences Tools and Resources
Protocols, spectra viewers, and product information from one of the leaders in the world of flow cytometry
Biolegend-Flow Cytometry Tools
Tools that can be utilized to optimize your experiments including spectra viewers and panel builders
Thermofisher Flow Cytometry
Vendor resources dedicated to flow cytometry
Expert Cytometry
Website dedicated to providing webinars, articles and protocols to advance the world of cytometry
Compensation Basics
An overview of compensation in flow cytometry by Mario Roederer
Publications
Guidelines for the Use of Flow Cytometry and Cell Sorting in Immunological Studies
A valuable historical resource for all things flow cytometry- protocols, sample prep, etc.
Flow Cytometry- A Basic Introduction
Easy to follow basic historical introduction to flow cytometry
Flow Cytometry Core contact information and authorization form
Maegan Capitano, PhD
Scientific Director
malcapit@iu.edu
Jim Ropa, PhD
Associate Director
jropa@iu.edu
Artur Plett, PhD
Lab Manager
pplett@iu.edu
