Shared Facilities

Multiplex Analysis Core

Shared Facilities

The Multiplex Analysis Core (MAC) offers microplate-based bioassay systems that can perform multiplex analysis of multiple different analytes in a single sample. Multiplex systems are faster, more efficient, and use less sample volume than other technologies such as ELISA and Western Blot. Off-the-shelf kits are available to detect analytes such as chemokines, cytokines, hormones, cell signaling molecules, phosphoproteins, or nucleic acids in areas such as inflammation, metabolism, cardiovascular disease, oncology, immunology, etc. Custom kits designed by the investigator are also a possibility.

The MAC uses a Bio-Plex 200 bead-based suspension system (Luminex platform) for analyte quantitation in the picogram level. Immunoassay kits are commercially available for human, mouse, bovine, canine, porcine, rat, and primate samples.

The MAC is located at R3-C335 (Walther Hall), 980 W. Walnut St., Indianapolis, IN 46202.

Bio-Plex 200 System

The Bio-Plex 200 Multiplex System with High Throughput Fluidics (HTF) is a suspension bead-based bioassay system that uses microscopic beads dyed with different ratios of two fluorochromes that emit a color spectrum unique to that fluorochrome ratio. Each bead is also tagged with an antibody specific to a certain target. Up to 100 differentially dyed beads can be used in a single multiplex assay, each conjugated to a different antibody. These bead-antibody conjugates are incubated with the target sample, washed, and then incubated with biotin-labeled detection antibodies (BIOT/Ab) specific for secondary epitopes on the target. Samples are washed and then incubated with a phycoerythrin (PE) labeled streptavidin reporter (SA/PE), washed, and resuspended for analysis. The fluorescence of the bead-antibody-target-BIOT/Ab-SA/PE complex is then read on the Bio-Plex by a red laser that identifies the specific bead by its fluorescent signature and a green laser that excites the PE dye, with the intensity of PE fluorescence signal indicating the level of target bound.