Expertise with in vitro assays
The Cellular Response Technologies Core (CRT) is a shared facility of the IU Simon Comprehensive Cancer Center that supports the development of pharmacological and cellular therapies for cancer. The core provides IU researchers with access to cutting-edge technologies and offers validated, highly reproducible in vitro and in vivo assays in angiogenesis, endothelial and hematopoietic biology, and multiparametric flow cytometry. These capabilities are applied to the study of normal physiology as well as patient-related disorders.
In addition to assay development and execution, CRT is dedicated to training scientists in the effective use of these technologies. The core performs a wide range of assays for research sample analysis and serves as a hub for consultation, education, and novel assay development for investigators within and beyond the IU School of Medicine and Indiana University.
The CRT is equipped with three IncuCyte S3 Live-Imaging Systems. The IncuCyte S3 instrumentation allows for the automated, extended, real-time assessment of cellular behavior under various experimental conditions. It has multiple capabilities of real-time quantitative analysis for multiple biological processes such as proliferation, apoptosis, angiogenesis, cell migration/invasion, immune function, 3D spheroid and organoid formation.
Multiplex analysis is also performed in the CRT with the Bioplex-200, a powerful bead-based suspension system (Luminex platform) for analyte quantitation in the picogram level. Immunoassay kits are commercially available for human, mouse, bovine, canine, porcine, rat, and primate samples.
For more information, contact CRT manager Emily Sims at ecwillar@iu.edu or 317-278-7232.
