Angiogenesis and Endothelial Progenitor CellServices

The Angiogenesis and Endothelial Progenitor Cell Core conducts validated and highly reproducible in vitro and in vivo angiogenesis assays which include:

In Vitro Assay Systems in Endothelial Cell Biology

  1. Endothelial Cell Isolation from Human Umbilical Veins
  2. Endothelial Progenitor Cell Isolation from Human Peripheral Blood
  3. Endothelial Progenitor Cell Clonogenic Assays
  4. Retroviral Transduction of Primary Human Endothelial Cells with Fluorescently Labeled Proteins or short interference RNAs (siRNAs)
  5. Endothelial Cell Proliferation Assays
  6. Endothelial Cell Migration Assays
  7. Tube Forming Assays with Human Endothelial Cells
    • Co-Culture Tube Forming Assays Using Transwell Systems
    • Stromal cell/endothelial cells
    • Tumor cells/endothelial cells
    • Inflammatory cells/endothelial cells
  8. Ex Vivo Aortic Outgrowth Model of Capillary Structures

In Vivo Assay Systems in Endothelial Cell Biology

  1. Mouse Matrigel Plug Model
  2. Confocal Imaging of Tumor Vasculature in Tumor Implants and Transgenic Mice
  3. Bone Marrow Contribution to Tumor Microenvironments Utilizing Chimeric Mice with green fluorescent-labeled bone marrow cells.
  4. Transplantation of Human Endothelial Progenitor Cells into Immunodeficient Mice