Shared Facilities

Translational Genomics

Services

Services provided by the Translational Genomics Core Laboratory include:

  • For nucleic acid preparation: isolation, quantification and qualification (if applicable) of either formalin-fixed paraffin-embedded (FFPE) or fresh tissue/cells.
  • For DNA: a range of activities from whole-genome genotyping to more focused studies using standard or custom single nucleotide polymorphism (SNP) performed on human samples. Results include clustering of samples, amplification and/or deletion of genes.
  • For RNA: gene expression profiling interrogates multiple whole genomes or focused sets of target genes across large sample populations. The gene expression profiling can be performed on either human or mouse samples. For partially degraded RNA, cDNA-mediated Annealing, Selection, extension and Ligation (DASL) assay is performed to obtain mRNA level expression. Results include dandogram and fold expression difference of particular mRNA with user-specified a-level or confidence interval. If investigators provide FFPE RNA to the core, the core prequalifies that the RNA provided is compatible with DASL assay.

If needed, samples can be stored in our facility for a specified period of time. At users’ request, the core provides raw data.