In Vivo Therapeutics Core
Policies & Procedures
Request for services
To request mice from the on-site breeding facility, fill out the appropriate online mouse request form. Contact Karen Pollok (firstname.lastname@example.org) for detailed information on in vivo services and pricing or to set up a consultation and study design meeting.
Select the appropriate form below:
- NSG (NOD/scid/IL2Rgnull)
- NSG 3 Cytokine (CMV-IL3, CSF2, KITLG)
- F1/BoyJ x C57BL/6J
Acknowledgment, authorship, collaboration
All research work performed by the IVT core should be acknowledged in all ensuing publications. Fees paid for services provided by the core should not negate the potential of co-authorship by core scientists. These acknowledgements and achievements are important for the existence and continued funding of the core facility. Given that many validation procedures require advanced intellectual involvement by the core’s scientists (examples listed below), it is expected that this acknowledgement will be in the form of co-authorship according to the guidelines for authorship recommended by the International Committee of Medical Journal Editors.IVT scientists performing one or more of the following tasks meet the criteria for authorship:
- Literature review and providing assistance in selecting/conceiving the experiments appropriate for the study goals
- Experimental design
- Assay development/optimization
- Data analysis and interpretation of data, or both
- Preparation of pertinent sections/figures for the manuscript and revisions to address reviewer concerns/questions
- Mere collection of data (e.g., flow cytometry) using PI-provided protocols that require no further optimization and/or trouble-shooting.
- Mere collection of in vivo study data (no data analysis or interpretation by core scientists.
- Irradiation and/or transplant of bone marrow cells
- Routine sample processing (e.g., serum/plasma separation, isolation of PBMCs from blood)
Distribution of mice for studies not implemented by core personnel:
We maintain the following strains:
NSG: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ
Boy J: B6.SJL-Ptprca Pepcb/BoyJ
C57BL/6J x B6.SJL-Ptprca Pepcb/BoyJ
NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ
Data generated by the core is kept on a secure server with access to core personnel only. Communication of data to clients will be done using a secure website on the IUSCC intranet. Each lab's folder is password protected and accessed only by lab and core personnel. Data or information (verbal and/or electronic) are not shared with any outside parties.
If a resolution cannot be reached at the core director level, the issue will be taken to the core advisory committee for discussion and resolution.
Cost recovery/payment policy:
If recovery of payment becomes an issue, the IUSCC business managers will contact the customer and/or their department to collect charges owed. If there is a dispute in charges, the conflict resolution policy above will be followed.
Prioritization of work:
Core studies or services will be prioritized based on IUSCC and/or IUSM membership followed by non members. Projects enter the Core upon receipt of a study request form and approved animal study or amendment. In the event that there is a backlog of studies, services will be prioritized based on IU Simon Cancer Center and/or IU School of Medicine membership followed by non-members. Priority will be given to users with peer-reviewed grant funding.
The core is funded, in part, by grants and contracts of the investigators who utilize the facility. All IUSCC members receive discounted rates. Due to the variety of in vivo models and drug regimens requested, the specific costs of a study will be calculated once the study design is finalized.
Weekend rates will be 2 times the regular procedure rate, with minimum charge per trip ($182)External rates TBD upon consultation.
|Service||Internal IU faculty rates|
|Tissue culture fees||$40.43/hour|
|Lentiviral transduction of cell line (includes cell transduction and flow cytometry verification)||$40.43/hour + flow lab fees|
|Methylene blue assay for cell viability after drug treatment||$40.43/hour|
|Tissue culture supply fees (tubes, pipettes, tips, trypsin, etc.)||$5.78/hour|
|Mycoplasma testing cell line (required for invivo studies)||$67.00/cell line|
|Cell line banking, authentication, distribution||Charges dependent on effort and supplies|
|Experimental technique training||$40.43/hour|
|Cost analysis and billing||$40.43/hour|
|Draft and submit study amendments||$40.43/hour|
|Drug formulation (15 min increments)||$40.43/hour|
|Surgical procedures/orthotic implant||$52.50/hour/person|
|NSG Cyto mice||$53.00/mouse|
|Boy J mice||$30.00/mouse|
|C57 / Boy J F1||$34.00/mouse|
|C57BL / 6J mice||$29.00/mouse|
|Biohazard mouse room per diems||$1.09/cage/day|
|Conventional mouse room per diems||$0.83/cage/day|
|Data entry, analysis and graphing||$80.86/week of study|
|Irradiation prior to experiment||$40.43/hour|
|Irradiation of cells||$40.43/hour|
|Irradiation “late” fee if >15 minutes late (with no written notice in advance)||$40.43 first occurrence + additional 10 percent for each subsequent occurrence|
|Tattoo fees (animal identification)||$2.31/mouse|
|Inhalation anesthesia fees (per hour to cover O2, isoflurane)||$10.61/hour|
|Palpate for tumors||$1.26/mouse|
|IP, ID, or IM injection||$2.54/mouse|
|Studies with staggered timing (dosing, bleeding, etc.)||$40.43/hour|
|Complete blood count (CBC)||$12.71/mouse|
|Survival or terminal bleed||$6.35/mouse|
|Euthanize and dispose (only)||$1.26/mouse|
|Euthanize + gross necropsy||$12.71/mouse|
|Euthanize + tumor (fixation)||$12.71/mouse|
|Euthanize + full necropsy||$19.56/mouse|
|Full necropsy + tissues in cassettes||
|Cellularity and progenitor analysis (BM or spleen)||$58.52/mouse **|
|Hematopoietic progenitor analysis (human UCB)||$38.20/drug treatment **|
|** This is for estimation purposes only. Acutal billed cost will include all needed supplies + actual effort|
|Weekend rates will be 2 times the regular procedure rate with minimum charge per trip ($182)|