Shared Facilities

Flow Cytometry

Services

Since the inception of this facility, there have been many requests for new services and new techniques have been added. The personnel and instrumentation available at the facility are capable of performing virtually any flow cytometric assay requested by IU Simon Cancer Center investigators.

Available instrumentation includes:

Multispectral Imaging Flow Cytometer:
  • Amnis Image StreamX MKII
Analyzers:
  1. BD FACSCalibur APC
    • 2 lasers (488, 630nm)
    • 2 light scatter parameters
    • 4 colors
  2. BD FACSCalibur no APC
    • 1 laser (488nm)
    • 2 light scatter parameters
    • 3 colors
  3. BD LSRII 407nm laser
    • 3 lasers (488, 630, 407nm)
    • 2 light scatter parameters
    • 9 colors
  4. BD LSRII 561nm laser
    • 3 lasers (488, 630, 561nm)
    • 2 light scatter parameters
    • 10 colors
Sorters:
  1. BD FACSAria
    • 3 lasers (407, 488, 630nm)
    • 2 light scatter parameters
    • 10 colors
  2. iCyt Reflection
    • Two sorting units (HAPS)
    • 3 lasers (488, 630nm, UV)
    • 9 colors

Available services include:

  1. Multiparameter (4, 5 or 6-color) immunofluorescence analysis and cell sorting
  2. DNA content and cell cycle analysis
  3. Simultaneous immunofluorescence and cell cycle analysis
  4. Single cell sorting
  5. Cell sorting (bulk or single cells) based on position of cells in cell cycle
  6. Apoptosis analysis (Annexin V/PI, Caspase, TUNEL and other assays)
  7. BrdU incorporation
  8. Viability assays
  9. Chromosome analysis (univariate and bivariate)
  10. Receptor - ligand interactions
  11. Kinetic analyses
  12. Off-line data analysis

For papers and grants, you may include the following addresses:

BD Biosciences
2350 Qume Drive
San Jose, CA  95131-1807

Sony Biotechnology Inc.
2100 S. Oak St.
Champaign, IL  61820

Amnis Corp. (part of EMD Millipore)
45 Elliott Ave. W., Suite 100
Seattle, WA 98119