Shared Facilities

Angio BioCore

Services

The Angio BioCore (ABC) is a state-of-the-art facility that has been established to conduct validated and highly reproducible in vitro and in vivo angiogenesis, hematopoietic and multi-parametric flow cytometry assays. These assays function as experimental platforms for understanding the basic mechanisms of hematopoiesis, angiogenesis and discovering compounds that inhibit new blood vessel formation in tumor microenvironments.

The specific duties of the ABC are:

  • Provide consultation and guidance for the design and analysis of experiments to define cell subsets that participate in angiogenesis (biomarkers for a variety of human diseases)
  • Prepare research and human clinical blood samples for acquisition in the Flow Cytometry Resource Facility and conduct detailed cell surface analysis using FlowJo software in ABC
  • Perform in vitro assays for angiogenesis
  • Perform in vitro clonal assays of hematopoietic progenitor cell function
  • Instruct interested investigators in the methods of hematopoietic stem and progenitor cell analysis and in a variety of angiogenesis assays
  • Develop new methods for detecting pro-angiogenic cells (non-vessel forming) and angiogenic cells (vessel-forming) from patient blood and tissue samples

Chargeback rates for services of the Angio BioCore, effective July 1, 2016, are below:

Cell Separation

Internal IU Faculty rates

External rates Services
$50 $75 Ficoll Preparation of Human Blood MNCs (20mls)
  • Preparation includes underlaying of up to 20mls of blood, washes and cell count
$110 $165 Ficoll Preparation of Human Blood MNCs (100mls)
  • Preparation includes underlaying of up to 100mls of blood, washes and cell count
$75 $113 CPT Preparation of Human Blood MNCs
  • Preparation includes processing of up to 48mls of blood in CPT tubes (supplied by the user), washes and cell count
$14 $21 Plasma Separation and Freeze Down
  • Preparation includes centrifuging and freeze down of up to eight vials of plasma
$14 $21 MNC Separation and Freeze Down (with 7C Flow)
  • Preparation includes freeze down of up to five vials of MNCs
$60 $90 MNC Separation and Freeze Down (without 7C Flow)
  • Preparation includes isolation of MNCs and freeze down of up to five vials of MNCs
$520 $780 Isolation of CD34+ Cells Via MACS Sort
  • Ficoli Separation of Cord Blood, Staining of MNCs with indirect CD34+ MACS kit
  • Cyopreservation of isolated cells per 1e6
  • Analysis of the cells for purity using FlowJo 1e6 cells
Cell Culture
Internal IU Faculty rates External rates Services
$40 $60 Quantitation of CFU-GEMMs from Human Blood
  • Plating, culture and quantitation of CFU-GEMMs (in triplicate)
  • Isolation of MNCs is a separate charge
$255 $383 Quantitation of Endothelial Outgrowth Cells (EOCs) from Human Blood
  • Plating, maintanence and quantitation of three wells for EOCs
  • Isolation of MNCs is a separate charge
$205 $308 Expansion and Cryopreservation of Endothelial Outgrowth Cells (EOCs)
  • Expansion of EOC colonies to passage 2 and cryopreservation of cells
Multi-Parametric Flow Cytometry
Internal IU Faculty rates External rates Services
$180 $270 7-Color MPFC Analysis of Human Blood MNCs
  • Staining of MNCs with seven antigen panel (CD14, CD16, CD31, CD34, CD45, AC133, LIVEDEAD), including necessary FMO controls and viability markers, and brief analysis in FlowJo
  • Does not include cost of data collection in Flow Cytometry Core
  • Isolation of MNCs is a separate charge
TBD TBD Up to 8-Color MPFC Analysis of Human Blood MNCs, Bone Marrow MNCs or Cell Lines

Can design any panel for the specific PI's interests

  • Staining of MNCs with eight antigen panel, including necessary FMO controls and viability markers, and brief analysis in FlowJo.
  • Does not include usage charge from Flow Cytometry Facility
  • Isolation of cells is a separate charge
Functional Assays
Internal IU Faculty rates External rates Services
$255 $383 Endothelial Cell Proliferation Assays (96 well format)
  • Thymidine incorporation in 96 well plates for up to 10 conditions (including controls) with 6 replicates
  • Does not include cost of cell line
$545 $818 Endothelial Cell Migration Assays
  • Haptotaxis in transwells for up to eight conditions (including controls) in triplicate
  • Does not include cost of cell line
$345 $518 Tube Forming Assays (Matrigel) with Human Endothelial Cells
  • Up to 10 conditions in triplicate assayed for endothelial cell tube formation
  • Does not include cost of cell line
$765 $1,148 Assessment of Endothelial Cell Apoptosis via TUNEL
  • TUNEL assessment of endothelial cell apoptosis
  • Up to six conditions in triplicate, including controls
  • Does not include cost of cell line.
Metabolism Studies/Seahorse XFp
Internal IU Faculty rates External rates Services
Cell Characterization, FCCP Titration $205 $308
Cell Glycolysis Assay-Stress Test $175 $263
Mitochondrial Respiration-Mito Stress Test $160 $240
Cell Energy Phenotype Assay $170 $255
Training for Equipment Use $235 $353
Assay Development $30/hour $30/hour
*The price of the assays include the cost of the Seahorse consumables and kits without any overhead charge.
Cryopreserved Cell Lines
Internal IU Faculty rates External rates Services
$235 $345 Human Umbilical Cord Blood EOCs
  • 1e6 cells
$235 $345 Human Peripheral Blood EOCs
  • 1e6 cells
$150 NA HUVECs
  • 1e6 cells
$300 NA HMVECs
  • 1e6 cells
$350 NA PMVECs
  • 1e6 cells
Miscellaneous
Internal IU Faculty rates External rates Services
$30/hour   Technician time
$60/hour   Retrospective analysis of FCS3.0 files
  • Data mining/analysis/re-analysis of previously acquired list mode data (FCS3.0 files) of EPC or CHSPC phenotyping for newly identified cell
    sub-populations of interest